| Therapeutic Potential of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells in Murine Model of Hepatic Schistosomiasis |
| Article 15, Volume 10, Issue 2, June 2020, Page 151-162 |
| Document Type: Original Article |
| DOI: 10.21608/AEJI.2020.26363.1061 |
| Authors |
| Mohammad Abd-El Rehiem Bebars1; Hanan Hussein Kamel1; Hala Gabr2; Heba Abdel Kader Aminou1; Lobna Shash3; Shimaa Essam Al-din Mohammed1; Ayman Ashkar   4 |
| 1Medical Parasitology department, Faculty of Medicine, Ain Shams University, Cairo, Egypt |
| 2Clinical Pathology department, Faculty of Medicine, Cairo University, Cairo, Egypt |
| 3Pathology department, Faculty of Medicine, Ain Shams University, Cairo, Egypt |
| 41st: Medical Parasitology department, Faculty of Medicine ,Ain Shams University, Cairo, Egypt. 2nd: college of medicine - University of Bisha - KSA |
| Abstract |
| Background and Aim: The pathogenesis of schistosomiasis is mainly due to the host’s immune response against Schistosoma eggs trapped in tissues causing a granulomatous reaction. Recently, stem cell therapy has drawn much attention due to their potential for self-renewal and differentiation. The present study was designed to investigate the regenerative and antifibrotic potential of Human Umbilical Cord Blood derived Mesenchymal Stem Cells (HUCB-MSCs) in hepatic granuloma due to schistosomiasis mansoni in the experimental murine model.
Material and Methods: Swiss Albino mice (n=70) were divided into 3 groups; Group A: 30 mice infected with S. mansoni cercariae and subjected to intrahepatic injection of HUCB-MSCs by a single dose of 1 million HUCB-MSCs at 12 weeks post infection (wpi), G (A1): 10 mice sacrificed at 16 (wpi), G (A2):10 mice sacrificed at 18 (wpi), G (A3): 10 mice sacrificed at 20 (wpi). Group B: 30 mice infected with S. mansoni cercariae (positive control), G (B1):10 mice sacrificed at 16 (wpi), G (B2):10 mice sacrificed at 18 (wpi), G (B3):10 mice sacrificed at 20 (wpi). Group C: 10 uninfected mice (negative control).
Results: Treatment with HUCB-MSCs, showed an improvement in the histopathological picture of the liver with diminution in the size of granulomas and the overall fibrotic content. Hyperplasic changes in intrahepatic biliary radicals obviously decreased in test groups.
Conclusion: The human umbilical cord blood-derived MSCs are promising and effective therapy for the treatment of diseased or damaged liver tissues. |
| Keywords |
| Cord blood-derived MSCs; Schistosomiasis mansoni; Liver fibrosis; Immunohistochemistry |
| Main Subjects |
| Endemic medicine |